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vector control  (Addgene inc)


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    Structured Review

    Addgene inc vector control
    Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+hygro+vector/pBABE-hygro+(Plasmid+%231765)/pm40754831-267-20-23
    Average 94 stars, based on 69 article reviews
    vector control - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Functional complexes between YAP2 and ZO-2 are PDZ domain-dependent, and regulate YAP2 nuclear localization and signalling1
    Article Snippet: .. YAP2-WT and several Flag–ZO-2 cDNAs in p2 × Flag-CMV2 vector were subcloned into pBABE-puro vector or pBABE-hygro vector (Addgene). ..

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: After lentivirus infection, IGROV1, MDA-MB-468 and OVCAR4 cells were selected with 1.5 μg/mL, 2 μg/mL, and 0.5 μg/mL of Puromycin (Sigma-Aldrich) respectively for 2-3 weeks and analyzed for loss of SLC1A3 by western blot. .. Human SLC1A3 was cloned into the pBABE-hygro vector (gift from Feng Zhang (Addgene plasmid # 1765; ): pBABE-hygro vector was digested with BamHI and SalI and cDNA coding for human SLC1A3 (purchased from IDT) cloned using In-Fusion HD cloning kit from Clontech (Takara Bio USA, Inc). ..

    Article Title: CRISPR/Cas9‐mediated knockout of HBsAg inhibits proliferation and tumorigenicity of HBV‐positive hepatocellular carcinoma cells
    Article Snippet: .. To establish stable LHBs‐Flag‐, MHBs‐Flag‐, and SHBs‐Flag‐overexpressing HCC cell lines, the LHB, MHB, and SHB genes were synthesized according to the sequences of hepatitis B virus (HBV 991) complete genome (GenBank accession no. AF461363) and cloned into pBABE‐hygro vector (Addgene; plasmid 1765). .. 293FT cells were then co‐transfected with LHBs‐Flag, MHBs‐Flag, SHBs‐Flag or its empty vector construct, and a packaging mix using Lipofectamine 3000 reagent (Invitrogen) to generate retroviral particles.

    Article Title: Regulation of GTPase function by autophosphorylation
    Article Snippet: .. Ectopic expression of mutant HA-K-RAS4B was done using the pBABE-hygro vector (Addgene plasmid #1765) ( ). ..

    Article Title: Reprogramming of human somatic cells to pluripotency with defined factors.
    Article Snippet: Pluripotency pertains to the cells of early embryos that can generate all of the tissues in the organism.. Embryonic stem cells are embryo-derived cell lines that retain pluripotency and represent invaluable tools for research into the mechanisms of tissue formation.. Recently, murine fibroblasts have been reprogrammed directly to pluripotency by ectopic expression of four transcription factors (Oct4, Sox2, Klf4 and Myc) to yield induced pluripotent stem (iPS) cells.

    Nuclear Magnetic Resonance:

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: .. Vectors encoding human ATF667 and S1P site-mutated GFP-ATF652 were obtained from Addgene (#11974 and #32956).52,67 Mouse standard form CD44, mouse LDLR, and NMR standard form CD44 were cloned in pBabe-hygro vector (Addgene #1765).68 Plasmids were transfected to cells using TransIT-2020 transfection reagent (Mirus). ..

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance
    Article Snippet: .. Vectors encoding human ATF6 and S1P site-mutated GFP-ATF6 were obtained from Addgene (#11974 and #32956)., Mouse standard form CD44, mouse LDLR, and NMR standard form CD44 were cloned in pBabe-hygro vector (Addgene #1765). .. Plasmids were transfected to cells using TransIT-2020 transfection reagent (Mirus).

    Clone Assay:

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: .. Vectors encoding human ATF667 and S1P site-mutated GFP-ATF652 were obtained from Addgene (#11974 and #32956).52,67 Mouse standard form CD44, mouse LDLR, and NMR standard form CD44 were cloned in pBabe-hygro vector (Addgene #1765).68 Plasmids were transfected to cells using TransIT-2020 transfection reagent (Mirus). ..

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: After lentivirus infection, IGROV1, MDA-MB-468 and OVCAR4 cells were selected with 1.5 μg/mL, 2 μg/mL, and 0.5 μg/mL of Puromycin (Sigma-Aldrich) respectively for 2-3 weeks and analyzed for loss of SLC1A3 by western blot. .. Human SLC1A3 was cloned into the pBABE-hygro vector (gift from Feng Zhang (Addgene plasmid # 1765; ): pBABE-hygro vector was digested with BamHI and SalI and cDNA coding for human SLC1A3 (purchased from IDT) cloned using In-Fusion HD cloning kit from Clontech (Takara Bio USA, Inc). ..

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance
    Article Snippet: .. Vectors encoding human ATF6 and S1P site-mutated GFP-ATF6 were obtained from Addgene (#11974 and #32956)., Mouse standard form CD44, mouse LDLR, and NMR standard form CD44 were cloned in pBabe-hygro vector (Addgene #1765). .. Plasmids were transfected to cells using TransIT-2020 transfection reagent (Mirus).

    Article Title: CRISPR/Cas9‐mediated knockout of HBsAg inhibits proliferation and tumorigenicity of HBV‐positive hepatocellular carcinoma cells
    Article Snippet: .. To establish stable LHBs‐Flag‐, MHBs‐Flag‐, and SHBs‐Flag‐overexpressing HCC cell lines, the LHB, MHB, and SHB genes were synthesized according to the sequences of hepatitis B virus (HBV 991) complete genome (GenBank accession no. AF461363) and cloned into pBABE‐hygro vector (Addgene; plasmid 1765). .. 293FT cells were then co‐transfected with LHBs‐Flag, MHBs‐Flag, SHBs‐Flag or its empty vector construct, and a packaging mix using Lipofectamine 3000 reagent (Invitrogen) to generate retroviral particles.

    Transfection:

    Article Title: CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance.
    Article Snippet: .. Vectors encoding human ATF667 and S1P site-mutated GFP-ATF652 were obtained from Addgene (#11974 and #32956).52,67 Mouse standard form CD44, mouse LDLR, and NMR standard form CD44 were cloned in pBabe-hygro vector (Addgene #1765).68 Plasmids were transfected to cells using TransIT-2020 transfection reagent (Mirus). ..

    Expressing:

    Article Title: ErbB2/Her2-dependent downregulation of a cell death-promoting protein BLNK in breast cancer cells is required for 3D breast tumor growth
    Article Snippet: SB203580 (Sigma–Aldrich, St. Louis, MO, USA), trastuzumab (Roche, Mississauga, ON, Canada), bortezomib Santa Cruz Biotechnology (Santa Cruz, CA, USA) Matrigel (VWR, Mississauga, ON, Canada), HBSS medium (Sigma–Aldrich). .. BLNK cDNA in pcDNA3.1 was from Genscript (Piscataway, NJ, USA). pBABE-hygro vector was from Addgene (Cambridge, MA, USA). pRetroX-TetOneTM-Puro expression vector was from Takara Bio (Mountain View, CA, USA). .. To generate pRetroX-TetOne™-Puro-BLNK expression vector the BLNK-encoding sequence was excised from pcDNA3.1 by EcoRI and BamHI restriction enzymes and inserted using T4 DNA ligase in EcoRI- and BamHI-digested pRetroX-TetOne™-Puro.

    Article Title: Regulation of GTPase function by autophosphorylation
    Article Snippet: .. Ectopic expression of mutant HA-K-RAS4B was done using the pBABE-hygro vector (Addgene plasmid #1765) ( ). ..

    Cloning:

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3
    Article Snippet: After lentivirus infection, IGROV1, MDA-MB-468 and OVCAR4 cells were selected with 1.5 μg/mL, 2 μg/mL, and 0.5 μg/mL of Puromycin (Sigma-Aldrich) respectively for 2-3 weeks and analyzed for loss of SLC1A3 by western blot. .. Human SLC1A3 was cloned into the pBABE-hygro vector (gift from Feng Zhang (Addgene plasmid # 1765; ): pBABE-hygro vector was digested with BamHI and SalI and cDNA coding for human SLC1A3 (purchased from IDT) cloned using In-Fusion HD cloning kit from Clontech (Takara Bio USA, Inc). ..

    Synthesized:

    Article Title: CRISPR/Cas9‐mediated knockout of HBsAg inhibits proliferation and tumorigenicity of HBV‐positive hepatocellular carcinoma cells
    Article Snippet: .. To establish stable LHBs‐Flag‐, MHBs‐Flag‐, and SHBs‐Flag‐overexpressing HCC cell lines, the LHB, MHB, and SHB genes were synthesized according to the sequences of hepatitis B virus (HBV 991) complete genome (GenBank accession no. AF461363) and cloned into pBABE‐hygro vector (Addgene; plasmid 1765). .. 293FT cells were then co‐transfected with LHBs‐Flag, MHBs‐Flag, SHBs‐Flag or its empty vector construct, and a packaging mix using Lipofectamine 3000 reagent (Invitrogen) to generate retroviral particles.

    Virus:

    Article Title: CRISPR/Cas9‐mediated knockout of HBsAg inhibits proliferation and tumorigenicity of HBV‐positive hepatocellular carcinoma cells
    Article Snippet: .. To establish stable LHBs‐Flag‐, MHBs‐Flag‐, and SHBs‐Flag‐overexpressing HCC cell lines, the LHB, MHB, and SHB genes were synthesized according to the sequences of hepatitis B virus (HBV 991) complete genome (GenBank accession no. AF461363) and cloned into pBABE‐hygro vector (Addgene; plasmid 1765). .. 293FT cells were then co‐transfected with LHBs‐Flag, MHBs‐Flag, SHBs‐Flag or its empty vector construct, and a packaging mix using Lipofectamine 3000 reagent (Invitrogen) to generate retroviral particles.

    Mutagenesis:

    Article Title: Regulation of GTPase function by autophosphorylation
    Article Snippet: .. Ectopic expression of mutant HA-K-RAS4B was done using the pBABE-hygro vector (Addgene plasmid #1765) ( ). ..



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